Anaerobic bacteria for biogas production

Biogas is an anaerobically produced ecofriendly renewable form of energy which can address the harmful effects associated with conventional source of fossil fuels. The objectives of present study were to examine effects of temperature, particle size and enzymes on biogas production.

Moose (Alces alces) thrives in woody browse in semi arctic region and it was presumed that it hosts unique micro-organisms capable of producing fibrolytic enzymes.

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An experiment was conducted in the laboratory of INN, Blæstad. Cow manure with 5% dry matter content and wheat straw with 3 different particle size were treated with 3 dose of Moose rumen bacteria (MRB) culture along with 3 temperature settings.

A cubical model was selected according to DoE (Design of experiments) and 11 treatments with different combinations of temperature, particle size and enzymes were tested. ml Erlenmeyer flasks were used as reactors, whereas gas was collected in ml syringes.

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